Why are gloves important for HPLC analysis?
HPLC columns (stationary phase) are expensive consumables that are sensitive to clogging. To maximise column life, it is essential to use the highest quality solvents available (HPLC/MS grade). It is also recommended to filter and degas the mobile phase.
The HPLC method involves handling and therefore the potential exposure of technicians to chemicals hazardous to human health.
This exposure can take place:
During the preparation of the mobile phases (solvents) used for HPLC analysis (filtration and degassing);
During the connection/change of solvent bottles (handling of tubing, risk of spillage/breakage when changing bottles);
During the sample preparation/injection phase.
When it comes to handling chemicals, the importance of proper hand protection cannot be overstated. Single-use gloves act as the first line of defence. However, not all gloves offer the same protection against chemicals.
Why is the performance of disposable gloves against chemicals important?
The HPLC method involves the use of a variety of solvents and chemicals, each with its own unique properties. The gloves used must therefore be compatible with these substances, to avoid their degradation and the accidental exposure of technicians to harmful chemicals. Opting for gloves that are specially adapted to resist the chemical being handled helps to guarantee operator safety.
What are the recommendations for selecting suitable HPLC gloves?
Choose gloves that are known to be resistant to the chemicals and compatible with the HPLC solvents you are handling.
Don’t forget to change your gloves regularly based on the breakthrough time given in the technical data sheet for your gloves, and at the very least, on the slightest contact with the chemical product.
Inspect the integrity of your gloves for any signs of damage or deterioration and replace them promptly if necessary.
Come and speak with a SHIELD Scientific rep at our stand to discuss further.
