Spraying device for the automated derivatization of TLC/HPTLC plates
The CAMAG Derivatizer sets a new standard of reproducibility and convenience in reagent transfer onto TLC/HPTLC plates by employing a unique “micro droplet” spraying technology (patent pending). The device is suitable for all common reagents.
In addition to the unsurpassed homogeneous reagent distribution, the Derivatizer offers other advantages as compared to manual spraying.
Environmentally friendly and safe handling through a closed system
User-independent results
Low reagent consumption, i.e. 4 mL for 20 x 20 cm plates and 3 mL for 20 x 10 cm plates
Intuitive operation and easy cleaning
Derivatization means another step in the process of planar chromatography, consequently causing an increase in variance. Even with a trained, expedient technician manually spraying a reagent, the relative standard deviation (RSD) of the measured values rises up to 12%. Using the CAMAG Derivatizer, the RSD increases by less than 5%, thus providing very reproducible results, equal to those obtained with the Chromatogram Immersion Device, yet consuming considerably less reagent.
The following most common reagents have been tested and validated by the CAMAG laboratory for use with the CAMAG Derivatizer:
• Sulfuric acid reagent (10 % in methanol)
• Anisaldehyde reagent
• Natural product reagent
• Polyethylene glycol solution
• Iodine solution (0.5% in ethanol)
• Dragendorff reagent
• Fast blue salt B reagent
• Ehrlich’s reagent
• Phosphomolybdic acid reagent
• Ninhydrin reagent
• Copper (II) sulfate reagent
• Aniline-diphenylamine-phosphoric acid reagent
• Vanillin reagent
• Potassium hydroxide solution (5% in methanol)
• Aqueous solutions (enzymatic solutions, etc.)
